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Title of Journal: Ann Bot

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Abbravation: Annals of Botany

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Narnia

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DOI

10.1016/0038-1101(78)90234-4

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0305-7364

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Cuticular Membrane Fine Structure of Nicotiana tab

Authors: KR ÜGER H VAN RENSBURG L PEACOCK J
Publish Date: 1996/01/01
Volume: 77, Issue: 1, Pages: 11-16
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Abstract

The cuticular membrane CM of Nicotiana tabacum L including the cellin wall CW was examined to gain more information about the nature and chemical constitution of its fine structure for possible inclusion in a model system as recent literature questions its function as a major water permeability barrier Different preparation techniques were used and the results evaluated to select a method for future studies on tobacco leaf cuticles Fixation with OsO 4 included in the primary fixative either as a vapour or in combination with other agents followed by OsO 4 as postfixative gave good contrast of the CM The lamellar structure of the tobacco cuticle proper CP was revealed by contrasting with uranyl acetate and lead citrate The fine lamellar structure of the CP was very clearly contrasted when KMnO 4 was included in the primary fixative This was interpreted as indicating the tobacco CP to be polar The reticulate fibrillar pattern of the tobacco cuticular layer CL containing polysaccharides was well contrasted when either OsO 4 or paraformaldehyde were included in the primary fixative Cold fixation with glutaraldehyde and dimethyl sulphoxide and postfixation with OsO 4 revealed electronopaque material in the outer cutinized irregularly outlined region of the CW These ultrahistochemical reactions are discussed in relation to the known chemical composition and possible water permeability of the CM

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  12. The Structure and Fungal Associates of Mycorrhizas in Leucopogon parviflorus (Andr.) Lindl.
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