Authors: Robbins T P Harbord R M Sonneveld T Clarke K
Publish Date: 2000/03/01
Volume: 85, Issue: suppl_1, Pages: 105-112
Abstract
The cultivated petunia Petunia hybrida has been a popular system in which to study genetic physiological and biochemical aspects of gametophytic selfincompatibility As with other members of the Solanaceae a number of SRNase genes have been isolated for functional Salleles We have identified SRNase sequences for two additional functional Salleles Sv and S3 These alleles are more similar to alleles from other families of the Solanaceae Nicotiana and Solanum than to any petunia alleles reported previously The total number of Salleles in P hybrida is at least ten in spite of its cultivated origin However most cultivars of P hybrida are in fact selfcompatible and this appears to arise from the prominence of a single previously described allele So The implications of this observation for the origin of selfcompatibility in P hybrida are discussed The Slocus of P hybrida has recently been mapped using an indirect method involving TDNA insertions Seven TDNA insertions that were previously shown to be closely linked to the Slocus were physically mapped on the long arm of chromosome III using fluorescent insitu hybridization The most tightly linked TDNA insertions are in a subcentromeric position This is consistent with the centric fragments of P inflata obtained by irradiation mutagenesis that carry additional Sloci and confer a pollenpart mutant phenotype An Slinked restriction fragment length polymorphism RFLP marker CP100 was used to confirm this chromosomal assignment and has provided evidence for Slocus synteny in the Solanaceae
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