Transcript profiling on developingEmphasis Type="

Authors: Filip Cnudde Chiaraluce Moretti Andrea Porceddu Mario Pezzotti Tom Gerats

Publish Date: 2003/06/27

Volume: 16, Issue: 2, Pages: 77-85

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Abstract

The cDNAAFLP transcript profiling technique was used to analyse gene expression during flower development in Petunia hybrida Reproductive and vegetative floral organs were sampled at five developmental stages and gene expression profiles were compared This allowed us to assemble an inventory of genes expressed mainly in anthers during microspore development and in ovaries during macrosporogenesis About 6000 transcript tags were generated 354 of which showed a modulated and/or organspecific expression pattern Stamenspecific transcripts exhibiting an upregulation in gene expression were well represented in our screening Ovaryspecific transcripts were less frequently observed and often displayed a constant level of gene expression Of 194 fragments characterised further by sequencing 35 showed homology with known genes in a database search They belong to a wide range of gene classes such as proteases transcription factors and genes involved in metabolism cell cycle and disease resistance The usefulness of cDNAAFLP transcript profiling as a tool to unravel complex developmental processes at the molecular level is discussed

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