Gene expression associated with apogamy commitment

Authors: Angela R Cordle Erin E Irish ChiLien Cheng

Publish Date: 2012/09/25

Volume: 25, Issue: 4, Pages: 293-304

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Abstract

Apogamy is a phenomenon in which a sporophyte develops asexually directly from a cell or cells of a gametophyte It is a phenomenon described mainly in lower plants but shares certain aspects with apomixis in angiosperms The genes involved in apogamy commitment in ferns are unknown We hypothesize that the mechanism of asexual reproduction is controlled in lower and higher plants by overlapping sets of genes To this end we created a normalized subtracted cDNA library that represents genes with increased expression during apogamy commitment in the fern Ceratopteris richardii The cDNA library consists of 170 unique sequences Compared to the mature gametophyte transcriptome of the fern Pteridium aquilinum the apogamy library is enriched in plant GOSlim terms that are associated with stress and metabolism In silico expression analyses of the closest Arabidopsis homologs of the apogamy library revealed many genes that display preferential expression in seed and flower tissues structures that are absent in ferns This apogamy library provides a rich resource for investigations into the genetic control of apogamy in ferns and comparisons with the asexual processes of higher plantsWe would like to thank J Fassler University of Iowa for critical readings of the manuscript and D Houston University of Iowa and G Hehman Carver Center for Genomics for assistance with the RTqPCR experiments and sequencing This work was supported by a grant from the University of Iowa Research Council ARC was also supported by Avis Cone Summer Fellowships

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