Development of a structured model for batch cultur

Authors: M A Del Nobile C Altieri M R Corbo M Sinigaglia E La Notte

Publish Date: 2003/07/09

Volume: 30, Issue: 7, Pages: 421-426

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Abstract

A combined stochasticdeterministic model able to predict the growth curve of microorganisms from inoculation to death is presented The proposed model is based on the assumption that microorganisms can experience two different physiological states nonproliferating and proliferating The former being the physiological state of the cells right after their inoculation into the new extracellular environment the latter the state of microorganisms after adaptation to the new medium To validate the model a Lactobacillus bulgaricus strain was tested in a medium at pH 46 at two different temperatures 42°C and 35°C Curves representing the bacterial growth cycle were satisfactorily fitted by means of the proposed model Moreover due to the mechanistic structure of the proposed model valuable quantitative information on the following was obtained rate of conversion of nonproliferating cells into proliferating cells growth and death rate of proliferating cells and rate of nutrient consumption

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