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Title of Journal: J IND MICROBIOL BIOTECHNOL

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Abbravation: Journal of Industrial Microbiology and Biotechnology

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Springer-Verlag

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10.1016/0278-6125(93)90267-w

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1476-5535

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Polyhydroxyalkanoate synthase genotype and PHA p

Authors: Daniel K Y Solaiman Vittoria Catara Sebastiana Greco
Publish Date: 2005/02/22
Volume: 32, Issue: 2, Pages: 75-82
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Abstract

A collection of Pseudomonas corrugata and P mediterranea strains two closely related species was evaluated for the presence and variability of pha loci Using PCR methods that specifically amplify segments of mediumchainlength polyhydroxyalkanoate mclPHA synthase genes we demonstrated the presence of phaC1 and phaC2 in all P mediterranea strains tested and in six out of 56 strains of P corrugata screened The remaining 50 strains of P corrugata yielded only the phaC2 subgene fragment on detection by a combined PCRrestriction endonuclease analysis method or a seminested PCRamplification approach A Southern hybridization study on a representative strain from this group however indicated the presence of the phaC1 gene Nucleic acid sequences of the subgene phaC fragments of the representative strains from the three groups showed an overall similarity ranging from 95 to 100 The major repeatunit monomers of the mclPHAs isolated from these selected strains are βhydroxyoctanoate 33–47 mol and βhydroxydecanoate 26–36 mol These results differentiate for the first time the strains of P corrugata into two phadistinguishable groups This study also documents for the first time the production of mclPHA in P mediterraneaThe authors thank Nicole Cross and Marshall Reed for their technical assistance This work was supported in part by funding from the Ministero dell’Istruzione dell’Università e della Ricerca Italy PON 20002006 “Utilization of waste material to develop biodegradable polymers PHA for agriculture and agroindustry” No 12842/01


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