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Title of Journal: Plant Soil

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Abbravation: Plant and Soil

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Kluwer Academic Publishers

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DOI

10.1002/chin.200745089

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ISSN

1573-5036

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A method for assessing arbuscular mycorrhizal fung

Authors: Mervyn Shepherd Linh Nguyen Megan E Jones J Doland Nichols F Lynn Carpenter
Publish Date: 2006/12/08
Volume: 290, Issue: 1-2, Pages: 259-268
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Abstract

We identified five taxonomic groups of arbuscular mycorrhizal fungi AMF inside roots of young trees of six species of legumes and six species of nonlegumes from a field site in southern Costa Rica using an AMF groupspecific PCR assay of the intergenic transcribed sequence and 18S rRNA gene fragment Assay specificity was verified by cloning and sequencing representatives from four of the five AMF groups We found no difference in overall AMF diversity levels between legumes and nonlegumes or between plant species Some groups of AMF may associate more frequently with legumes than others as Glomus Group A Glomus mosseae/intradices group representatives were detected more frequently in legumes than nonlegumes relative to Glomus Group B Glomus etunicatum/claroideum representativesThe authors thank Eduer Sandi Tapia Rolando Mendez Rodriguez and Leonel Figueroa Vargas for their conscientious assistance in the field work We thank F Eliott for technical assistance in the laboratory Financial support was provided by University of California Research Expeditions Program and Rainforest Agrarian International Network

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