Hepatoprotective effect of total flavonoids from

Authors: Yihang Wu Fang Wang Qunxiong Zheng Longxi Lu Hongtian Yao Changxin Zhou Xiumei Wu Yu Zhao

Publish Date: 2006/03/18

Volume: 13, Issue: 4, Pages: 569-578

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Abstract

The hepatoprotective activities of total flavonoids of Laggera alata TFLA were evaluated by carbon tetrachloride CCl4induced injury in primary cultured neonatal rat hepatocytes and in rats with hepatic damage In vitro TFLA at a concentration range of 1–100  upmug/ml improved cell viability and inhibited cellular leakage of two enzymes hepatocyte aspartate aminotransferase AST and alanine aminotransferase ALT caused by CCl4 In vivo oral treatment with TFLA at doses of 50 100 and 200 mg/kg significantly reduced the levels of AST ALT total protein and albumin in serum and the hydroxyproline and sialic acid levels in liver Histopathological examinations revealed that liver damage were improved when treated with TFLA Meanwhile 11diphenyl2picrylhydrazyl DPPH and superoxide radicals scavenging activities of TFLA were also determinated To understand the exact components of TFLA responsible for the hepatoprotective effect nine flavonoid compounds were isolated and identified from TFLA In conclusion the present investigation was the first to verify the hepatoprotective effect of L alata in vitro and in vivo The hepatoprotective action of TFLA is likely related to its potent antioxidative and antiinflammatory activity Neutralizing reactive oxygen species by nonenzymatic mechanisms and enhancing the activity of original natural hepaticantioxidant enzymes may be the main mechanisms of TFLA against CCl4induced injury

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