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Title of Journal: J Comput Aided Mol Des

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Abbravation: Journal of Computer-Aided Molecular Design

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Kluwer Academic Publishers

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1573-4951

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Design and characterization of an engineered gp41

Authors: Kent D Stewart Kevin Steffy Kevin Harris John E Harlan Vincent S Stoll Jeffrey R Huth Karl A Walter Emily GramlingEvans Renaldo R Mendoza Jean M Severin Paul L Richardson Leo W Barrett Edmund D Matayoshi Kerry M Swift Stephen F Betz Steve W Muchmore Dale J Kempf Akhter Molla
Publish Date: 2007/02/09
Volume: 21, Issue: 1-3, Pages: 121-130
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Abstract

Two new proteins of approximately 70 amino acids in length corresponding to an unnaturallylinked N and Chelix of the ectodomain of the gp41 protein from the human immunodeficiency virus HIV type 1 were designed and characterized A designed tripeptide links the Cterminus of the Chelix with the Nterminus of the Nhelix in a circular permutation so that the Chelix precedes the Nhelix in sequence In addition to the artificial peptide linkage the Chelix is truncated at its Nterminus to expose a region of the Nhelix known as the “TrpTrpIle” binding pocket Sedimentation crystallographic and nuclear magnetic resonance studies confirmed that the protein had the desired trimeric structure with an unoccupied binding site Spectroscopic and centrifugation studies demonstrated that the engineered protein had ligand binding characteristics similar to previously reported constructs Unlike previous constructs which expose additional shallow nonconserved and undesired binding pockets only the single deep and conserved TrpTrpIle pocket is exposed in the proteins of this study This engineered version of gp41 protein will be potentially useful in research programs aimed at discovery of new drugs for therapy of HIVinfection in humansThis manuscript is dedicated to Yvonne Connolly Martin The precepts of molecular properties and architectures promulgated by Yvonne in 41 years at Abbott extend even to the level of protein design All of the authors thank her for being a steadfast source of inspiration and scientific rolemodel of the highest degree Crystallographic data were collected at beamline 17ID in the facilities of the Industrial Macromolecular Crystallography Association Collaborative Access Team IMCACAT at the Advanced Photon Source These facilities are supported by the companies of the Industrial Macromolecular Crystallography Association

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