Journal Title
Title of Journal: Physiol Mol Biol Plants
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Abbravation: Physiology and Molecular Biology of Plants
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Publisher
Springer-Verlag
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Authors: S A Ranade T S Rana D Narzary
Publish Date: 2009/05/14
Volume: 15, Issue: 1, Pages: 61-70
Abstract
We are interested in studying the distribution and range of diversity amongst the pomegranates in India Single Primer Amplification Reaction SPAR profiling using Random Amplified Polymorphic DNA RAPD and Directed Amplification of Minisatellite DNA DAMD methods enabled the determination of the genetic diversity amongst a total of 64 Indian pomegranate genotypes including 15 wild 34 semiwild and 14 cultivated types SPAR profile data were scored for the computation of pairwise distances as well as a Neighbour Joining NJ tree of all the genotypes Eight RAPD and four DAMD primers showed discrete polymorphic patterns amongst these genotypes From the profiles obtained with all the 12 primers considered together 259 bands were scored The NJ tree generated after a 1000 bootstrap test using Jaccard coefficient showed separation of Lagerstroemia speciosa used as the outgroup taxon while the pomegranate genotypes were resolved into distinct genetic lineages such that all the cultivated except CBd70 and wild genotypes except W101 clearly separated from other genotypes in distinct sub clusters while the semiwild genotypes were resolved into three subclusters The greatest and least distances detected between genotypes were 094 and 012 097 and 024 and 095 and 038 amongst the cultivated semiwild and the wild genotypes respectively The results indicate the high levels of genetic diversity present amongst the genotypes Significantly the wild genotypes also have a reasonably good range of diversity A good germplasm collection especially including the wild genotypes will enable a better pomegranate improvement program Both SPAR methods RAPD and DAMD are found to be useful for studying the genetic diversity of pomegranate
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