Assessing genetic diversity among six populations

Authors: Khushboo Sethi Priyanka Siwach Surender Kumar Verma

Publish Date: 2015/10/24

Volume: 21, Issue: 4, Pages: 531-539

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Abstract

Among the four cultivated cotton species G hirsutum allotetraploid presently holds a primary place in cultivation Efforts to further improve this primary cotton face the constraints of its narrow genetic base due to repeated selective breeding and hence demands enrichment of diversity in the gene pool G arboreum diploid species is an invaluable genetic resource with great potential in this direction Based on the dispersal and domestication in different directions from Indus valley different races of G arboreum have evolved each having certain traits like drought and disease resistance which the tetraploid cotton lack Due to lack of systematic race wise characterization of G arboreum germplasm it  has not been explored fully During the present study 100 polymorphic SSR loci were  used to genotype 95 accessions belonging to 6 races of G arboreum producing 246 polymorphic alleles mean number of effective alleles was 1505 AMOVA showed 14  of molecular variance among population groups 34  among individuals and remaining 52  within individuals UPGMA dendrogram based on Nei’s genetic distance distributed the six populations in two major clusters of 3 populations each race ‘bengalense’ was found more close to ‘cernuum’ than the others The clustering of 95 genotypes by UPGMA tree generation as well as PCoA analysis clustered ‘bengalense’ genotypes into one group along with some genotypes of ‘cernuum’ while rest of the genotypes made separate clusters Outcomes of this research should be helpful in identifying the genotypes for their further utilization in hybridization program to obtain high level of germplasm diversityFinancial support was provided by the University Grants Commission UGC Ministry of Human Resource Development Government of India New Delhi India in the form of Major Research Project to Priyanka Siwach Principal Investigator and Surender Kumar Verma CoPrincipal Investigator The authors are grateful to the Director CICR Sirsa and Director CICR Nagpur for providing the germplasm We also acknowledge the infrastructural facilities provided by Chaudhary Devi Lal University Sirsa Haryana India for all the lab work Acknowledgement is also extended to CICR Sirsa for allowing the use of experimental field facility and polyhouse facility

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