Contribution of transgenic Emphasis Type="Italic"

Authors: Sergio Svistoonoff Hassen Gherbi Mathish NambiarVeetil Chonglu Zhong Zuzanna Michalak Laurent Laplaze Virginie Vaissayre Florence Auguy Valérie Hocher Patrick Doumas Jocelyne Bonneau Didier Bogusz Claudine Franche

Publish Date: 2009/12/04

Volume: 50, Issue: 1-2, Pages: 3-11

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Abstract

The Casuarinaceae family is a group of 96 species of trees and shrubs that are tolerant to adverse soil and climatic conditions In the field Casuarinaceae bears nitrogenfixing root nodules so called actinorhizal nodules resulting from infection by the soil actinomycete Frankia The association between Casuarina and Frankia is of tremendous ecological importance in tropical and subtropical areas where these trees contribute to land stabilization and soil reclamation During differentiation of the actinorhizal nodule a set of genes called actinorhizal nodulins is activated in the developing nodule Understanding the molecular basis of actinorhizal nodule ontogenesis requires molecular tools such as genomics together with gene transfer technologies for functional analysis of symbiotic genes Using the biological vectors Agrobacterium rhizogenes and A tumefaciens gene transfer into the two species Allocasuarina verticillata and Casuarina glauca has been successful Transgenic Casuarinaceae plants proved to be valuable tools for exploring the molecular mechanisms resulting from the infection process of actinorhizal plants by FrankiaThis work was supported by the Institut de Recherche pour le Développement IRD and by the Agence Nationale de la Recherche ANR Blanc project NewNod ANR06BLAN0095 Dr Mathish NambiarVeetil was financially supported by a grant from the Department of Biotechnology Ministry of Science and Technology Government of India Pr Chonglu Zhong was supported by a DSF Département Soutien et Formation fellowship from IRD

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