Journal Title
Title of Journal: Symbiosis
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Publisher
Springer Netherlands
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Authors: Karita Negandhi Patricia L Blackwelder Alexander V Ereskovsky Jose V Lopez
Publish Date: 2010/05/11
Volume: 51, Issue: 1, Pages: 117-129
Abstract
Sponges can filter large volumes of seawater and accumulate highly diverse and abundant microbial communities within their tissue Cultureindependent techniques such as fluorescent in situ hybridization FISH 16S small subunit SSU rRNA gene analyses and transmission electron microscopy TEM were applied to characterize the presence and distribution of microbes within sponges abundant on south Florida reefs This study found that coral diseaseassociated bacteria CDAB are harbored within Agelas tubulata and Amphimedon compressa FISH probes detected several potential bacterial pathogens such as Aurantimonas coralicida Cytophaga sp Desulfovibrio spp Serratia marcescans and Vibrio mediterranei within A compressa and A tubulata host sponges Spatial differences in the distribution of targeted bacteria were seen within sponge hosts Transmission electron microscopy of A compressa indicated there was a higher concentration of bacteria in the choanosome compared to the ectosome These observed spatial distributions support the presence of internal sponge niches which could play a role in the location of the CDAB within the spongesThis publication is a result of funding from the National Oceanic and Atmospheric Administration Center for Sponsored Coastal Ocean Science under awards NA07NOS4000200 to Nova Southeastern University for the National Coral Reef Institute The authors wish to thank Husain Alsayegh from the University of Miami Center for Advanced Microscopy UMCAM for his expertise in electron microscopy and Abby Renegar for assistance with histological sectioning for FISH analyses Jeffrey Prince was kind enough to permit use of the TEM in his lab at the Biology Department at the University of Miami This work was in part supported from a President’s Faculty Research and Development Grant PB and JL from Nova Southeastern University The authors also are grateful to Dr Kim Ritchie and Dr Steve Monday for helpful comments on earlier versions of the manuscript
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