Authors: Hong Liu Deqian Jiang Shebing Zhang Baiqing Ou
Publish Date: 2009/12/08
Volume: 24, Issue: 1, Pages: 17-24
Abstract
Twentyone male ApoE gene knockout mice were randomized into three groups to receive either placebo in addition to normal mice chow n = 7 placebo in addition to a highfat diet n = 7 or aspirin 58 mg/kg/d in addition to a highfat diet n = 7 After 12 weeks of study the mice were euthanized and serum cholesterol thromboxane B2 and 6 textketo textPGtextF 1alpha were examined Fractalkine and cyclooxygenase expression in aorta were measured and the atherosclerotic lesion analyzedPathology image analysis showed that the atherosclerotic plaque was the most extensive in the highfat diet group while the addition of aspirin greatly reduced the severity of the plaque Both RTPCR analysis and immunohistochemical analysis showed that fractalkine expression was the strongest in the highfat diet group and was significantly decreased by aspirin treatment Serum thromboxane B2 was lowered by aspirin while 6 textketo textPGtextF 1alpha and cholesterol remained unchanged
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