A panel of polymorphic microsatellite markers in H

Authors: Thakur Mukesh Ishwari D Rai Rishi P Mandhan Sambandam Sathyakumar

Publish Date: 2011/01/18

Volume: 57, Issue: 4, Pages: 983-989

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Abstract

Isolation and development of new microsatellite markers for any species is still labourintensive and requires substantial inputs of time money and expertise Therefore crossspecies microsatellite amplification can be an effective way in obtaining microsatellite loci for closely related taxa in bird species We have reported microsatellite loci for Himalayan monal for the first time Fifteen microsatellite markers developed for chicken were crossamplified in Himalayan monal All the tested 15 microsatellite markers were polymorphic with mean ± se allelic number of 4 ± 151 ranging 2–7 per locus The observed heterozygosity in the population ranged between 0285 and 0714 with mean ± se of 0499 ± 0125 indicating considerable genetic variation in this population While 12 loci conformed to Hardy–Weinberg equilibrium P  005 3 loci ie MCW0295 MCW0081 MCW0330 deviated from it P  005 No evidence for linkage disequilibrium was observed among pair of loci Our study show that these 15 microsatellites loci could be employed in population genetic studies for Himalayan monal and their applicability in Jungle Bush Quail Grey francolin and Kalij pheasantThe study was the part of a national project on the Conservation Genetics of the Red Junglefowl Gallus gallus that is funded by the Wildlife Institute of India Dehradun India We thank the Director Dean and Dr SP Goyal WII for their encouragement and support We thank the Chief Wildlife Warden Uttarakhand Divisional Forest Officers and field staff of Kedarnath Wildlife and Shivalik Reserved Forest Divisions Uttarakhand who helped us during the field study At WII We thank Sudhanshu Mishra Lalit K Sharma and Manish Bhardwaj for the valuable discussions on the concept and Bibek Yumnan for providing samples of Jungle Bush quail and Grey francolin We thank the two anonymous reviewers for their valuable comments on the earlier version of this manuscript

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