Control of Drug Loading and Release Properties of

Authors: Claudia Blüm Thomas Scheibel

Publish Date: 2012/02/02

Volume: 2, Issue: 2, Pages: 67-74

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Abstract

The controlled delivery of watersoluble substances is one important issue in pharmaceutical and medical applications Biocompatible polymers which can easily be processed in an all aqueous process with controllable and adjustable properties have been thoroughly investigated in the past for their use as drug delivery vehicles Recently we established submicroparticles produced from the engineered spider silk protein eADF4C16 as potential carriers for highly watersoluble drugs Here we investigate the influence of crosslinking on the structural integrity of the submicroparticles and the effect on drug loading and release To analyze the orderofaddition influences of processing of submicroparticles on drug loading and release we tested five different preparation routes We showed that the preparation route largely influences the loading capacity of the eADF4C16 submicroparticles In the preferred preparation route rhodamine B and the protein are coprecipitated by saltingout yielding the highest loading Further crosslinking the proteins with APS ammonium persulfate and Rubpy Tris22′ bipyridyldichlororutheniumII has an impact on loading as well as on the release behavior of drug molecules as shown exemplarily with rhodamine BThis work was supported by the Bundesministerium für Bildung und Forschung BMBF grant number 13N11340 We gratefully thank Lukas Eisoldt and Andrew Smith for proof reading and Felix Bauer Lukas Eisoldt Anja Hagenau Andrew Smith Michael Suhre and Stefanie Wohlrab for critical comments on the manuscript We would like to thank Nicolas Helfricht for assistance with the zeta potential measurements

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