Journal Title
Title of Journal: BioNanoSci
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Abbravation: BioNanoScience
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Authors: A Lateef M A Azeez T B Asafa T A Yekeen A Akinboro I C Oladipo F E Ajetomobi E B GueguimKana L S Beukes
Publish Date: 2015/10/06
Volume: 5, Issue: 4, Pages: 196-205
Abstract
In this study seed and seed shell extracts of Cola nitida were investigated for the biogenic synthesis of silver nanoparticles AgNPs under ambient condition The biosynthesized AgNPs were characterized through visual development of colour UV–vis spectroscopy Fourier transform infrared spectroscopy FTIR and transmission electron microscopy TEM The antibacterial activities of the AgNPs were determined using some multidrugresistant clinical isolates The biosynthesized AgNPs depicted brown and yellowish orange colour using seed and seed extract respectively with maximum absorbance readings at 4575 and 4545 nm The FTIR spectrum showed strong peaks at 329249 208698 and 163178 cm−1 for seed extractmediated AgNPs while peaks of 330213 208605 and 163371 cm−1 were obtained for seed shell extractmediated AgNPs all indicating that proteins were the capping and stabilization molecules in the biogenic synthesis of AgNPs The AgNPs were spherical in shape with sizes ranging from 8 to 50 and 5 to 40 nm for seed and seed shellmediated AgNPs respectively The energydispersive Xray EDX analysis showed the presence of silver as a prominent metal while the selected area electron diffraction SAED pattern conformed to the facecentred cubic phase and crystalline nature of AgNPs At various concentrations ranging from 50–150 μg/ml the AgNPs inhibited growth of multidrugresistant strains of Klebsiella granulomatis Pseudomonas aeruginosa and Escherichia coli to the tune of 10–32 mm Comparatively seed shell extractmediated AgNPs had better activities with minimum inhibitory concentration MIC of 50 μg/ml against all the tested isolates while the MICs of seed extractmediated AgNPs were obtained as 50 80 and 120 μg/ml against E coli P aeruginosa wound and P aeruginosa burn respectively This study has demonstrated the feasibility of ecofriendly biogenic synthesis of AgNPs using seed and seed shell extracts of C nitida and the report to the best of our knowledge is the first reference to extracts of C nitida for the green synthesis of AgNPs
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