Authors: Otto Hyink Frans Laas Peter K Dearden
Publish Date: 2012/12/07
Volume: 44, Issue: 3, Pages: 306-313
Abstract
The honeybee haplodiploid sex determination system depends on genetic variation at the complementary sexdeterminer csd locus In closed populations of honeybees especially those undergoing selective breeding the number of csd alleles can drop such that brood viability is affected Here we present two polymerase chain reaction tests that allow the discrimination of csd alleles Such tests should find utility in bee breeding programmes allowing the tracking and maintenance of csd alleles through successive generationsWe would like to thank the Tertiary Education Commission’s Partnering for Innovation Scheme and the Agricultural and Marketing Research and Development Trust for funding this work We would like to thank the National Research Centre for Growth and Development for funding the purchase of realtime PCR equipment and dnature http//dnatureconz for testing this protocol We would also like to thank Betta Bees Research Ltd for access to their closed population of bees and the National Beekeepers Association of New Zealand for their support
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