Journal Title
Title of Journal: Apidologie
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Authors: Eva Vianey AlquisiraRamírez José Roberto ParedesGonzalez Víctor Manuel HernándezVelázquez José Augusto RamírezTrujillo Guadalupe PeñaChora
Publish Date: 2014/05/13
Volume: 45, Issue: 6, Pages: 707-718
Abstract
Fiftyfour Bacilluslike strains were isolated from dead Varroa destructor collected in 24 colonies of bees from seven apiaries The total proteins of 22 isolates were evaluated against adult female V destructor 100 μg/mL but only nine caused over 80 mortality The LC50 of EA261 EA3 and EA113 isolates were calculated The most virulent isolate was EA261 as 967 of the mites were killed 36 h after treatment and the LC50 was 150 μg/mL EA3 produced 933 mite mortality after 48 h treatment with an LC50 of 71 μg/mL Finally EA113 produced 933 mite mortality after 60 h treatment with an LC50 of 228 μg/mL Additionally the EA3 and EA261 isolates were used to determine mortality on adults and larvae of worker bees of A mellifera When the 16S rRNA gene was amplified and sequenced the three strains were found to belong to the species B thuringiensis The results showed that honey bee adults and larvae are not negatively affected by identified B thuringiensis isolates indicating that they could be successfully used for the biological control of V destructor without adverse effects on adults and larvae of A mellifera and reduce the negative impact of this mite on colonies
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