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Title of Journal: Polar Biol

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Abbravation: Polar Biology

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Springer Berlin Heidelberg

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DOI

10.1016/0016-5085(92)90854-r

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ISSN

1432-2056

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Metabolic fingerprinting of arctic copepods Empha

Authors: Bjørn Henrik Hansen Kristin Degnes Ida Beathe Øverjordet Dag Altin Trond R Størseth
Publish Date: 2013/08/08
Volume: 36, Issue: 11, Pages: 1577-1586
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Abstract

Recent development in highthroughput unbiased analytical tools transcriptomics proteomics and metabolomics has opened the possibility to assess a multitude of molecular endpoints in organisms In the present work we used a combination of metabolomics tools proton nuclear magnetic resonance spectroscopy 1HNMR and liquid chromatography coupled with mass spectrometry LC–MS and multivariate statistical analysis to identify speciesspecific metabolome fingerprints and speciesspecific metabolites in the three Arctic copepods Calanus finmarchicus C glacialis and C hyperboreus Principal component analysis separated the three species with high specificity and sensitivity and some speciesspecific metabolites were putatively annotated These tools can be used for future studies within basal biology systems biology bioprospecting and ecotoxicology As a supplementary analytical tool to genetic analyses speciesspecific metabolites have a potential to be used to separate closely related Arctic Calanus species from net hauls

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  9. Heat hardening in Antarctic notothenioid fishes
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