Incidence and characterization of Emphasis Type="

Authors: Fatemeh ShamsaddenSaeed Hossain Massumi Sakineh Moradi Mohammad Maddahian Jahangir Heydarnejad Akbar Hosseini Pour Arvind Varsani

Publish Date: 2013/12/01

Volume: 25, Issue: 1, Pages: 78-84

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Abstract

A survey was carried out to determine the extent of Potato virus V PVV infection together with other potyviruses in Iran in both commercial and local potato varieties We found a low incidence of PVV in commercial varieties compared to a local potato cultivar Zardi in which we noted a PVV infection up to ~329  We determined the genomic sequence 9812 nucleotides of one isolate KERLALP from cultivar Agria and the 3′terminal sequence including coat protein CP gene of four additional isolates from cultivar Zardi The Iranian isolate PVV KERLALP was found to share 91  sequence identity with the Scottish isolate DV42 AJ243766 of PVV The CP gene sequences of the PVV isolates from Iran shared 965 to 993  pairwise nucleotide identity and they shared 975  pairwise identity with the CP sequences of all other PVV isolates available in public databases Our host studies indicated that the Iranian PVV isolate had a narrower host range and infected Nicotiana debneyi and N glutinosa test plants Within the commercial varieties of potato in Iran we noted a significant amount of mixed potyvirus infection This study is the first report of occurrence and complete genome of PVV in Iran

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