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Title of Journal: VirusDis

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Abbravation: VirusDisease

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Springer India

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10.1016/0029-554x(60)90136-1

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2347-3517

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Inducible expression of magnesium protoporphyrin c

Authors: Sachin Ashok Bhor Chika Tateda Tomofumi Mochizuki KenTaro Sekine Takashi Yaeno Naoto Yamaoka Masamichi Nishiguchi Kappei Kobayashi
Publish Date: 2017/01/27
Volume: 28, Issue: 1, Pages: 69-80
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Abstract

Recent studies with Y satellite RNA Ysat of cucumber mosaic virus have demonstrated that Ysat modifies the disease symptoms in specific host plants through the silencing of the magnesium protoporphyrin chelatase I subunit CHLI which is directed by the Ysat derived siRNA Along with the development of peculiar yellow phenotypes a drastic decrease in CHLItranscripts and a higher accumulation of Ysat derived siRNA were observed To investigate the molecular mechanisms underlying the Ysat—induced chlorosis especially whether or not the reduced expression of CHLI causes the chlorosis simply through the reduced production of chlorophyll or it triggers some other mechanisms leading to the chlorosis we have established a new experimental system with an inducible silencing mechanism This system involves the expression of artificial microRNAs targeting of Nicotiana tabacum CHLI gene under the control of chemically inducible promoter The CHLI mRNA levels and total chlorophyll content decreased significantly in 2 days enabling us to analyze early events in induced chlorosis and temporary changes therein This study revealed that the silencing of CHLI did not only result in the decreased chlorophyll content but also lead to the downregulation of chloroplast and photosynthesisrelated genes expression and the upregulation of defenserelated genes Based on these results we propose that the reduced expression of CHLI could activate unidentified signaling pathways that lead plants to chlorosisAuthors thank Kazue Obara for technical assistance This study was supported in part by The United Graduate School of Agricultural Sciences Ehime University and JSPS KAKENHI Grants 26292026 and 15K14664 to Kobayashi Bhor has been supported by Rotary Yoneyama Memorial Foundation for doctoral studies


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