Journal Title
Title of Journal: Limnology
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Authors: Yuko Sugiyama Patrick G Hatcher Rachel L Sleighter Tomoyo Suzuki Chizuru Wada Tetsu Kumagai Osamu Mitamura Toshiya Katano Shinichi Nakano Yuji Tanaka Valentin V Drucker Vladimir A Fialkov Masahito Sugiyama
Publish Date: 2014/02/15
Volume: 15, Issue: 2, Pages: 127-139
Abstract
To elucidate the molecular characteristics of dissolved organic matter DOM in Lake Baikal 3D excitationemission matrix spectroscopy and Fourier transform ion cyclotron resonance mass spectrometry FTICR MS were employed From the linear relationship between the humiclike peak intensities excitation/emission = 305 nm/430 nm and dissolved organic carbon DOC concentrations in the water samples extending from the Selenga River mouth to offshore central lake in the south basin allochthonous DOM appeared to be a main contributor to the DOC concentrations However DOM with fewer fluorophores dominated in the South Basin of the lake at stable DOC concentrations of ca 084 mg C l−1 Meanwhile FTICR MS analysis and subsequent principal component analysis across the transect revealed a transition of compounds with high Hdeficiency aromatic to compounds with low Hdeficiency aliphatic that dominate pelagic openlake water We believe that this molecular change is induced by photodegradation which mainly alters aromatic compoundsWe are grateful to the captain and crew of the R/V Treskov for assistance with sample collection We are also grateful to S Hatcher College of Sciences Major Instrumentation Cluster Facility Old Dominion University for assistance with FT ICRMS and Dr K Hayakawa Lake Biwa Environmental Research Institute for assistance with 3D EEMs measurements The work was also supported by the National Science Foundation CHE0089147 USA and GrantsinAid for Scientific Research from the Ministry of Education Culture Sports Science and Technology MEXT Japan no 18255001
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