Expression pattern of Emphasis Type="Italic"Chla

Authors: Shaoshuai Liang Xiaoshi Ma Tiantian Han Dandan Yang Zhifeng Zhang

Publish Date: 2015/07/23

Volume: 14, Issue: 4, Pages: 731-738

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Abstract

The SOX2 protein is an important transcription factor functioning during the early development of animals In this study we isolated a fulllength cDNA sequence of scallop Chlamys farreri sox2 Cfsox2 which was 2194 bp in length with a 981 bp open reading frame encoding 327 amino acids With realtime PCR analysis it was detected that Cfsox2 was expressed in unfertilized oocytes fertilized eggs and all the tested embryos and larvae The expression level increased significantly P 001 in embryos from 2cell to blastula and then decreased significantly P 001 and reached the minimum in umbo larva Moreover location of the Cfsox2 expression was revealed using whole mount in situ hybridization technique Positive hybridization signal could be detected in the central region of unfertilized oocytes and fertilized eggs and then strong signals dispersed throughout the embryos from 2cell to gastrula During larval development the signals were concentrated and strong signals were restricted to 4 regions of viscera mass in veliger larva In umbo larva weak signals could be detected in regions where presumptive visceral and pedal ganglia may be formed The expression pattern of Cfsox2 during embryogenesis was similar to that of mammal sox2 which implied that CfSOX2 may participate in the regulation of early development of C farreri

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