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Title of Journal: Int J Biometeorol

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Abbravation: International Journal of Biometeorology

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Springer Berlin Heidelberg

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DOI

10.1002/chin.199718175

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1432-1254

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Expression analysis of NOS family and HSP genes du

Authors: Vijay Pratap Yadav Satyaveer Singh Dangi Vikrant Singh Chouhan Mahesh Gupta Saroj K Dangi Gyanendra Singh Vijay Prakash Maurya Puneet Kumar Mihir Sarkar
Publish Date: 2015/07/24
Volume: 60, Issue: 3, Pages: 381-389
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Abstract

Approximately 50 genes other than heat shock protein HSP expression changes during thermal stress These genes like nitric oxide synthase NOS need proper attention and investigation to find out their possible role in the adaptation to thermal stress in animals So the present study was undertaken to demonstrate the expressions of inducible form type II NOS iNOS endothelial type III NOS eNOS constitutively expressed enzyme NOS cNOS HSP70 and HSP90 in peripheral blood mononuclear cells PBMCs during different seasons in Barbari goats Realtime polymerase chain reaction western blot and immunocytochemistry were applied to investigate messenger RNA mRNA expression protein expression and immunolocalization of examined factors The mRNA and protein expressions of iNOS eNOS cNOS HSP70 and HSP90 were significantly higher P  005 during peak summer and iNOS and eNOS expressions were also observed to be significantly higher P  005 during peak winter season as compared with moderate season The iNOS eNOS cNOS HSP70 and HSP90 were mainly localized in plasma membrane and cytoplasm of PBMCs To conclude data generated in the present study indicate the possible involvement of the NOS family genes in amelioration of thermal stress so as to maintain cellular integrity and homeostasis in goats

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