The beneficial effects of multispecies Emphasis T

Authors: Anita Talib Kwong Kok Onn Md Arif Chowdury Wan Mustaffa Wan Din Khairun Yahya

Publish Date: 2016/10/14

Volume: 25, Issue: 2, Pages: 849-866

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Abstract

Mud crabs Scylla are promising aquaculture crustaceans due to their fast growth and good market demand High rates of larval mortalities associated with bacterial diseases in hatcheries are one of the major obstacles to the growth of Scylla aquaculture Currently antibiotics are used extensively in Scylla larval culture to improve survival rate but its use poses numerous risks The aim of this study was to determine the culture performance of Scylla paramamosain larvae with the use of a probiotic namely a multispecies Bacillus at various concentrations In this study the effects of Bacillus spp were tested in three trials There were higher average larval survivals in treatments with a multispecies Bacillus probiotics added compared with the control especially at concentrations of 100 × 104 1233–1833 ± 176–376  compared to 167–367 ± 167–176  P  005 and 200 × 104 cfu/ml This was significantly lower than the control 133 ± 133 cfu/ml compared to 3267 ± 1105 cfu/ml during the later days posthatching Green colonies Vibrios are known to be pathogenic to Scylla larvae and thus lower numbers are desirable in culture The recommended concentration of the multispecies Bacillus to be added into S paramamosain larvae was therefore 100 × 104 and 200 × 104 cfu/ml 050 and 100 g/50 l respectively The use of Bacillus as a probiotic is an ideal alternative to antibiotics in Scylla hatcheries to provide a consistent commercially feasible production of seedlingsThe authors would like to thank Jocelyn E W Goon our temporary Project Assistant under our Short Term Grant We acknowledge the staff and students of CEMACS USM including the staff of “The Mud Crab Research Project” CEMACS This study was funded by the APEX Delivering Excellence 1002/PPANTAI/910331 USM Short Term Grant 304/PPANTAI/6312131 USM and the Post Graduate Research Grant Scheme USM The financial support of MyBrain15 Ministry of Higher Education and Graduate Assistant USM are equally acknowledged

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