Expression of the CuZn superoxide dismutase of As

Authors: Hamilton Andrew John Holdom Mary Denise Jeavons Lisa

Publish Date: 1996/06/01

Volume: 14, Issue: 2-3, Pages: 95-102

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Abstract

Andrew John Hamilton Mary Denise Holdom Lisa Jeavons Expression of the CuZn superoxide dismutase of Aspergillus fumigatus as determined by immunochemistry and immunoelectron microscopy FEMS Immunology Medical Microbiology Volume 14 Issue 23 June 1996 Pages 95–102 https//doiorg/101111/j1574695X1996tb00275xA polyclonal antibody against purified CuZn superoxide dismutase SOD from the pathogen Aspergillus fumigatus was raised in a sheep This antibody recognised purified A fumigatus SOD together with a single band of 19 kDa in A fumigatus cytoplasmic antigen by immunodevelopment of Western blots The polyclonal serum did not recognise either the manganese or iron containing forms of the enzyme however it was reactive against putative CuZn SODs in other members of the genus Aspergillus Immunofluorescent staining of A fumigatus cultures demonstrated expression of the CuZn SOD in conidia and hyphae with the cell wall staining particularly intensely Conidiophores were stained in an uniformly intense pattern Immunoelectron microscopy confirmed that the SOD was present within the hyphal cell wall although there was also labelling in the cytoplasm SOD may protect Aspergillus against oxidants produced by immune effector cells and these observations demonstrate that the enzyme is available to perform its antioxidant function within the cell wall

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