Influence of survivintargeted siRNA on the biolog

Authors: Ying Xiong Wen Guo Ting Li Ke Li

Publish Date: 2007/07/01

Volume: 1, Issue: 3, Pages: 304-307

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Abstract

The transient transfection of survivintargeted siRNA to Lovo cells and its influence on the biological features were studied Two pairs of 19 base pairs bp siRNAspecific targeted survivin gene were designed and synthesized by in vitro transcription Survivin1 Survivin2 After transient transfection of the two survivintargeted siRNAs to Lovo cells by Lipofectamine™ 2000 the expression of survivin mRNA was detected by reverse transcriptionpolymerase chain reaction RTPCR Apoptosis was detected by flow cytometry and cell proliferation was evaluated by MTT assay We found that the expression levels of survivin mRNA of the two RNAi groups Survivin1 group and Survivin2 group respectively decreased by 70 and 391 compared with the control Lovo’s Seventytwo hours after transfection apoptosis rates of the two RNAi groups were 2151 and 2628 both of which were higher than control Lovo’s 903 The results at 72 h after transfection were that the optical density OD at 490 nm of the two RNAi groups was 0581 ± 0070 and 0681 ± 0104 both of which were much lower than the control Lovo’s 2060 ± 0272 Based on the results we can draw a conclusion that the two survivintargeted siRNAs successfully suppressed the expression of survivin mRNA inhibited cell growth and induce cell apoptosis It provides a powerful evidence for colorectal carcinoma gene therapy

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