MSI/LOH and extron expression of the FHIT gene in

Authors: Yuping Xiao Lili Mao Chengbo Han Jinyi Li Lei Xu Yan Xin

Publish Date: 2007/02/01

Volume: 1, Issue: 1, Pages: 99-103

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Abstract

We detected loss of heterozygosity LOH and microsatellite instabilities MSI as well as extron expression of the fragile histidine triad FHIT gene in gastric carcinoma GC in order to evaluate their association with clinicopathological processes in gastric carcinogenesis LOH and MSI of the FHIT were detected by using PCR at 4 microsatellite loci D3S 1300 D3S 4103 D3S 1481 D3S 1234 in cancer tissues from 50 patients with primary GC with normal mucosa acting as matched controls FHIT transcripts were detected by nested RTPCR in 30 cases of GC and their products were sequenced Results show that the average frequencies of LOH and MSI of the FHIT gene in GC were 324 and 264 respectively There was no correlation between LOH and MSI of the FHIT gene in GC and the histological characteristics of gastric carcinoma Bormann’s or Lauren’s classification LOH of the FHIT gene in GC was related to depth invasiveness and its frequency in GC where serosa was penetrated was significantly higher than that in GC without serosa penetration 735 vs 375 P 005 The frequency of MSI in GC without lymph node metastasis was significantly higher than that in GC with lymph node metastasis 667 vs 343 P 005 Aberrant transcripts were found in 11/30 GC tissues Sequencing analysis of the aberrant fragments found a RTPCR product missing exons 5–7 in one case of GC and another product missing exons 4–7 Four of 10 400 cases of primary GC showed absent or decreased expression of the FHIT protein as compared to their matched normal tissues The findings in this study suggest that LOH and MSI of FHIT gene may induce aberrant extron expression which might play a role in gastric carcinogenesis

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