Journal Title
Title of Journal: Glycoconj J
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Abbravation: Glycoconjugate Journal
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Authors: Suzannah A Williams Pamela Stanley
Publish Date: 2008/09/05
Volume: 26, Issue: 3, Pages: 335-347
Abstract
The glycan epitopes termed stagespecific embryonic antigens SSEA occur on glycoproteins and glycolipids in mammals However it is not known whether these epitopes are attached to N or Oglycans on glycoproteins and/or on glycolipids in the developing mouse embryo In this paper the expression of the antigens SSEA1 SSEA3 SSEA4 and LeY was examined on ovulated eggs early embryos and blastocysts lacking either complex and hybrid Nglycans or core1 derived Oglycans In all cases antigen expression determined by fluorescence microscopy of bound monoclonal antibodies to embryos at the stage of development of maximal expression was similar in mutant and control embryos Thus none of these developmental antigens are expressed solely on either complex N or core 1derived Oglycans attached to glycoproteins in the preimplantation mouse embryo Furthermore neither of these classes of glycan is essential for the expression of SSEA1 SSEA3 SSEA4 or LeY on mouse embryosWe thank Wen Dong for excellent technical assistance and Dr Jason Aglipay for help with some experiments This work was supported by grant RO1 30645 from the National Institutes of Health to P S and received partial support from the Albert Einstein Cancer Center grant PO1 13330
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