Journal Title
Title of Journal: Glycoconj J
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Abbravation: Glycoconjugate Journal
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Authors: Anshuman Yadav Sneh Lata Singh Bhawna Yadav Sneha Sudha Komath
Publish Date: 2014/08/13
Volume: 31, Issue: 6-7, Pages: 497-507
Abstract
GPI2 encodes for one of the six accessory subunits of the GPINacetylglucosaminyltransferase GPIGnT complex that catalyzes the first step of GPI biosynthesis in S cerevisiae and C albicans It has been previously reported in S cerevisiae that this subunit physically interacts with and negatively modulates Ras signaling On the other hand studies from our lab have shown that the homologous subunit in C albicans is a positive modulator of Ras signaling Are the functions of this subunit therefore strictly species dependent We present here functional complementation studies on GPI2 from S cerevisiae and C albicans that were carried out to address this issue Expression of CaGPI2 in a ScGPI2 conditional lethal mutant could not restore its growth defects Likewise ScGPI2 overexpression in a CaGPI2 heterozygous mutant could not restore its deficient GPIGnT activity or reverse defects in its cell wall integrity and could only poorly restore filamentation However interestingly ScGPI2 could restore lanosterol demethylase CaERG11 levels and reverse azole resistance of the CaGPI2 heterozygote It appeared to do this by regulating levels of another GPIGnT subunit CaGPI19 which we have previously shown to be involved in crosstalk with CaERG11 Thus the effect of CaGPI2 on sterol biosynthesis in C albicans is independent of its interaction with the GPIGnT complex and Ras signaling pathways In addition the interaction of Gpi2 with other subunits of the GPIGnT complex as well as with Ras signaling appears to have evolved differently in the two organismsThis work was supported by funding from Department of Biotechnology DBT to SSK Partial funding by DBTBUILDER programme to SSK is also acknowledged We thank Dr Marwan Al Shawi for YEpHIS vector and Prof Alistair JP Brown for pACT1GFP vector SLS and BY were supported by Junior and Senior Research fellowships from ICMR and CSIR while Anshuman received support from the DBT project to SSK BY would also like to thank DSTPURSE for a shortterm fellowship Microscopy and RTPCR and facilities were used at the Central Instrumentation Facility School of Life Sciences Jawaharlal Nehru University
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