Journal Title
Title of Journal: Fish Sci
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Abbravation: Fisheries Science
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Authors: Tomomasa Matsuyama Chihaya Nakayasu Takamitsu Sakai Norihisa Oseko
Publish Date: 2009/01/23
Volume: 75, Issue: 2, Pages: 335-341
Abstract
Two monoclonal antibodies MAbs JFW1 and JFW10 were produced against peripheral blood leukocytes PBL in Japanese flounder Additionally MAbs against flounder immunoglobulin Ig JFW20 and JFW21 were generated for the surface marker of Ig+ leukocytes using purified serum Ig as an antigen MAb JFW1 recognized the surface marker of granulocytes and monocytes and MAb JFW10 specifically bound to the surface antigen of thrombocytes Flow cytometric analysis of PBL incubated with JFW1 JFW10 JFW20 and JFW21 revealed that 25–74 237–501 252–261 and 52–83 of all leukocytes were positive for these markers Analysis of head kidney leukocytes HKL showed that JFW1 JFW10 JFW20 and JFW21 bound to 305–363 19–28 64–83 and 19–30 of all leukocytes respectively Western blot analysis after SDSPAGE showed that JFW10 recognizes a protein of 115 kDa from lysed PBL JFW20 recognized the 70 and 74 kDa proteins of the heavy chain of Ig from serum No band was observed for either JFW1 or JFW21 These antibodies will be useful for the identification and isolation of Japanese flounder leukocyte subpopulations and will facilitate immunological studies of flounder
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Other Papers In This Journal:
- Pinger affects fish catch efficiency and damage to bottom gill nets related to bottlenose dolphins
- Development of the musculature and muscular abnormalities in larval seven-band grouper Epinephelus septemfasciatus
- Maturation process and reproductive biology of female Arabesque greenling Pleurogrammus azonus in the Sea of Japan, off the west coast of Hokkaido
- Effects of hypoxia on the feeding intensity and somatic condition of the blackfin flounder Glyptocephalus stelleri in Funka Bay, Japan
- Evaluation of the pigmentation stages and body proportions from the glass eel to yellow eel in Anguilla japonica
- Ecosystem structure and trophic dynamics of an exploited ecosystem of Bay of Bengal, Sundarban Estuary, India
- Potential use of corn co-products in fishmeal-free diets for juvenile Nile tilapia Oreochromis niloticus
- Isolation and characterization of 16 polymorphic microsatellite markers from Nibea albiflora
- The current propagation systems and physiological studies of Japanese chum salmon
- Molecular cytogenetic study of genome ploidy in the German mirror carp Cyprinus carpio
- Physiological response of the eye to different colored light-emitting diodes in Japanese flying squid Todarodes pacificus
- Growth, nutrient utilization, oxidative condition, and element composition of juvenile red sea bream Pagrus major fed with fermented soybean meal and scallop by-product blend as fishmeal replacement
- Cloning and nutritional regulation of polyunsaturated fatty acid desaturase and elongase of a marine teleost, the nibe croaker Nibea mitsukurii
- Interregional differences in mortality of aquacultured yellowtail Seriola quinqueradiata in relation to a Chattonella bloom in the Yatsushiro Sea, Japan, in 2010
- Understanding the accumulation features of POPs in squid from the offshore waters of southeast Korea
- The effect of lunar cycle, tidal condition and wind direction on the catches and profitability of Japanese common squid Todarodes pacificus jigging and trap-net fishing
- Vortex flow produced by schooling behavior of arabesque greenling Pleurogrammus azonus
- Lipid characteristics of coastal migratory Sarda orientalis tissues
- Reproductive cycle of the venerid clam Meretrix lusoria in Ariake Sound and Tokyo Bay, Japan
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