Journal Title
Title of Journal: Fish Sci
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Abbravation: Fisheries Science
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Authors: YaJuan Li YangChun Gao He Zhou Bo Liu Min Gao YuSheng Wang XiaoWen Sun
Publish Date: 2014/07/08
Volume: 80, Issue: 5, Pages: 963-968
Abstract
We examined the polyploidy of Cyprinus carpio the German mirror carp Specimens were collected in the field in Hulan China and treated with phytohemagglutinin and colchicine before the chromosome spreads and the karyotype of kidney cells were examined using silver staining chromomycin A3 CMA3/distamycin DA/46diamidino2phenylindole DAPI staining and fluorescence in situ hybridization FISH with a 58S + 28S rDNA probe One to twosilver stained Ag nucleolus organizing regions NORs were detected during metaphase and interphase events with 80 of the metaphase spreads and 69 of the interphase nuclei showing two AgNORs signals One CMA3 signal was detected on the terminus of the short arm of each submetacentric chromosome 8 SMC8 homolog n = 2 The 58S + 28S rDNA probe hybridized at the terminus of the short arm of each SMC8 homolog n = 2 The results of the AgNORs CMA3/DA/DAPI and 58S + 28S rDNA FISH analyses were consistent with regard to the total number and location of the SMC8 NORs in the chromosome spreads and karyotype indicating that at the molecular cytogenetic level the German mirror carp is an evolutionary tetraploid with two sets of chromosomes after diploidization
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