Actions and Comparative Efficacy of Phosphatidylch

Authors: A Gupta C Lobocki S Singh M Robertson O A Akadiri G Malhotra I T Jackson

Publish Date: 2009/02/07

Volume: 33, Issue: 3, Pages: 346-352

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Abstract

Phosphatidylcholine formulation has been used to dissolve local fat deposits This study aimed to evaluate and compare the effects of phosphatidylcholine formulation and its vehicle sodium deoxycholate alone on different cell lines to understand better its mechanism of actionAfter 24 h cells were exposed in 34 5dimethylthiazol2yl2 3diphenyl tetrazolium bromide reagent MTT assays to increasing dosages of phosphatidylcholine formulation 00156–05 mg/ml or an equivalent vehicle sodium deoxycholate solution pH 90 00066–0210 mg/ml Viability was assessed after 1 2 and 3 days of treatment Fat tissue 4 × 4 cm obtained ex vivo from the dorsal fat pads of five rabbits was injected with 2 ml of phosphatidylcholine formulation 50 mg/ml sodium deoxycholate 21 mg/ml or normal saline and incubated for 24 h These were examined histologically to identify cell lysis and morphologic changesAt 0125 and 025mg/ml doses of phosphatidylcholine solution CADMEC and HSkMC were more sensitive P  0001 oneway ANOVA than adipocytes at all time points examined Phosphatidylcholine formulation at a dose of 05 mg/ml and the equivalent vehicle sodium deoxycholate at a dose of 021mg/ml both induced nearly 100 fat cell lysis after 24 h and evidence of cell lysis as early as 6 h after exposure After incubation of fat tissue for 24 h with phosphatidylcholine formulation loss of intracellular lipid staining with an increase in extracellular lipids was seenIsolated sodium deoxycholate was almost as effective as the phosphatidylcholine formulation at clinical concentrations in reducing the viability of mature adipocytes over time Similar cytotoxic effects of phosphatidylcholine formulation on normal foreskin fibroblasts endothelial cells and human skeletal muscle cells also were observed The data prove that the formulation acts in a nonspecific manner and that its unintentional administration to other tissues causes cell death

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