Authors: Nils T Nyberg Mette Olaf Nielsen Jerzy W Jaroszewski
Publish Date: 2010/07/10
Volume: 6, Issue: 4, Pages: 489-496
Abstract
1H NMR metabolic profiles of urine from sheep exposed to prenatal nutritional restriction n = 19 and a control group with normal prenatal nutritional requirements n = 19 followed by either conventional n = 10 + 10 or high carbohydrate high fat postnatal diet n = 9 + 9 were studied Urine was sampled from 2 6 19 and 24monthold animals receiving differential dietary treatments during the first 6 months and the same normal diet later Principal component analysis of 1H NMR spectra n = 164 showed a Vshaped metabolic trajectory as a function of age and diet starting with urines with a high amount of glucose indicative of monogastriclike metabolism and exhibiting concomitant increase of metabolites related to rumen microflora mainly glycine conjugates of benzoic and phenylacetic acid as the ruminal metabolism developed Urines from young 2monthold animals exposed to prenatal undernutrition followed by normal postnatal diet showed metabolic patters that are ahead in time on the metabolic trajectory relative to the prenatal control group No longterm effects of fetal undernutrition alone or in combination with postnatal hypernutrition were observedFinancial support from the Danish Strategic Research Council grant no 2101050030 and a fellowship from the Alfred Benzon Foundation to the corresponding author are gratefully acknowledged We thank Dr Dan Stærk for participation in a preliminary phase of this work and Malin P Tygesen Anna H Kongsted Sanne M Husted Vibeke G Christensen Ruth Jensen Dennis S Jensen Vibeke B Hansen and Lennart E Carlsen who helped at various stages of the project with the handling of animals and urine sampling
Keywords: