Authors: Christiaan A Rees Katherine V Nordick Flavio A Franchina Alexa E Lewis Elizabeth B Hirsch Jane E Hill
Publish Date: 2017/01/12
Volume: 13, Issue: 2, Pages: 18-
Abstract
Microorganisms catabolize carboncontaining compounds in their environment during growth releasing a subset of metabolic byproducts as volatile compounds However the relationship between growth media and the production of volatile compounds has been largely unexplored todateHeadspace volatiles produced by cultures of K pneumoniae after growth to stationary phase in four rich media brain heart infusion broth lysogeny broth MuellerHinton broth and tryptic soy broth were analyzed using comprehensive twodimensional gas chromatography timeofflight mass spectrometry GC×GCTOFMS Differences in the composition of headspace volatiles as a function of growth media were assessed using hierarchical clustering analysis HCA and principal component analysis PCAA total of 365 volatile compounds were associated with the growth of K pneumoniae across all media of which 36 10 were common to all growth media and 148 41 were specific to a single medium In addition utilizing all K pneumoniaeassociated volatile compounds strains clustered as a function of growth media demonstrating the importance of media in determining the metabolic profile of this organismThis work was supported by National Institutes of Health grant R21 AI121076 to JE Hill and Lennart KA Lundblad Dartmouth College holds an Institutional Program Unifying Population and Laboratory Based Sciences award from the Burroughs Wellcome Fund and CA Rees was supported by this grant Grant1014106 KV Nordick was supported by the Paul K Richter and Evalyn E Cook Richter Memorial Fund awarded through Dartmouth College AE Lewis was supported by the Presidential Scholarship awarded through Dartmouth College We thank Mavra Nasir and Theodore R Mellors for their aid in editing this manuscript
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