Authors: Banthita Sawasdee HeinzR Köhler
Publish Date: 2010/08/14
Volume: 19, Issue: 8, Pages: 1487-1495
Abstract
We investigated the effects of metal ions on the embryonic development of the ramshorn snail Marisa cornuarietis by exposing embryos to varying concentrations of copper 0 50 100 and 250 μg Cu2+/L lead 0 5 10 and 15 mg Pb2+/L lithium 0 1 25 and 3 mg Li+/L or palladium 0 50 100 and 500 μg Pd2+/L Effects of these metals were examined by recording mortality the rate of tentacles and eyes formation heart rate hatching success and weight after hatching Compared to the control we found a significant delay in the formation of tentacles and eyes after treatment with 100 μg Cu2+/L 15 mg Pb2+/L 25 mg Li+/L or 500 μg Pd2+/L The heart rate decreased significantly at 500 μg Pd2+/L At 10 mg Pb2+/L 25 mg Li+/L or 500 μg Pd2+/L hatching was delayed significantly 50 μg Cu2+/L induced a significantly earlier hatching and reduced body weight The LC50 values were calculated to be about 50 μg Cu2+/L 500 μg Pd2+/L 2500 μg Li+/L and 10000 μg Pb2+/L These results show that the embryonic development of M cornuarietis is about as sensitive to copper and lithium compared to the most sensitive fishes used in embryo toxicity testing Even though the MariETT is a laboratorybased assay focusing on toxicological endpoints of a selected model species future application is envisaged to include testing of “natural” samples such as stream water or sediment interstitial water
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