Authors: Ye Yang Yao Mu XianChun Zeng Weiwei Wu Jie Yuan Yichen Liu E Guoji Feng Luo Xiaoming Chen Hao Li Jianing Wang
Publish Date: 2017/03/01
Volume: 26, Issue: 4, Pages: 490-501
Abstract
Hot Springs have unique geochemical features Microorganismsmediated arsenite oxidation is one of the major biogeochemical processes occurred in some hot springs This study aimed to understand the diversities of genes and microorganisms involved in arsenite oxidation from the outlet of an untraversed hot spring located at an altitude of 4226 m Microcosm assay indicated that the microbial community from the hot spring was able to efficiently oxidize AsIII using glucose lactic acid yeast extract or sodium bicarbonate as the sole carbon source The microbial community contained 7 phyla of microorganisms of which Proteobacteria and Firmicutes are largely dominant this composition is unique and differs significantly from those of other described hot springs Twenty one novel arsenite oxidase genes were identified from the samples which are affiliated with the arsenite oxidase families of αProteobacteria βProteobacteria or Archaea this highlights the high diversity of the arseniteoxidizing microorganisms from the hot spring A cultivable arseniteoxidizer Chelatococcu sp GHS311 was also isolated from the sample using enrichment technique It can completely convert 750 mg/L AsIII into AsV in 18 days at 45 °C The arsenite oxidase of GHS311 shares the maximal sequence identity 847 to that of Hydrogenophaga sp CL3 a nonthermotolerant bacterium At the temperature lower than 30 °C or higher than 65 °C the growth of this strain was completely inhibited These data help us to better understand the diversity and functional features of the thermophilic arseniteoxidizing microorganisms from hot springs
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