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Title of Journal: Ecotoxicology

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Abbravation: Ecotoxicology

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Springer US

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DOI

10.1007/s11426-009-0157-1

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1573-3017

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Estrogen alters the profile of the transcriptome i

Authors: Kun Lei Ruizhi Liu Lihui An Yingfeng Luo Gerald A LeBlanc
Publish Date: 2014/11/15
Volume: 24, Issue: 2, Pages: 330-338
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Abstract

We evaluated the transcriptome dynamics of the freshwater river snail Bellamya aeruginosa exposed to 17βestradiol E2 using the Roche/454 GSFLX platform In total 41869 unigenes with an average length of 586 bp representing 36181 contigs and 5688 singlets were obtained Among them 1808 3685 and 2547  matched sequences in the GenBank nonredundant nucleic acid database nonredundant protein database and Swiss protein database respectively Annotation of the unigenes with gene ontology and then mapping them to biological pathways revealed large groups of genes related to growth development reproduction signal transduction and defense mechanisms Significant differences were found in gene expression in both liver and testicular tissues between control and E2exposed organisms These changes in gene expression will help in understanding the molecular mechanisms of the response to physiological stress in the river snail exposed to estrogen and will facilitate research into biological processes and underlying physiological adaptations to xenoestrogen exposure in gastropods

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