Journal Title
Title of Journal: Transition Met Chem
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Abbravation: Transition Metal Chemistry
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Publisher
Springer Netherlands
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Authors: Hidetaka Torigoe Akira Ono Tetsuo Kozasa
Publish Date: 2010/12/08
Volume: 36, Issue: 2, Pages: 131-144
Abstract
Single nucleotide polymorphisms SNPs are base differences in the human genome These differences are favorable markers for genetic factors including those associated with risks of complex diseases and individual responses to drugs When two duplex DNAs with different types of SNPs are mixed and reannealed the two novel heteroduplexes containing mismatched base pairs are formed in addition to the two initial perfectly matched homoduplexes Heteroduplex analysis recognizing the newly formed mismatched base pairs is useful for SNP detection Various strategies to detect the mismatched base pairs were devised due to the potential applications of SNPs However they were not always convenient and accurate Here we propose a novel strategy to detect the mismatched base pairs by the specific interaction between the Hg2+ ion and a TT mismatched base pair and that between the Ag+ ion and a CC mismatched base pair UV melting indicated that the melting temperature of only the heteroduplexes with the TT and CC mismatched base pair specifically increased on adding the Hg2+ and Ag+ ion respectively Fluorescence resonance energy transfer analyses indicated that the intensity of fluorophore emission of only the fluorophore and quencherlabeled heteroduplexes with the TT and CC mismatched base pair specifically decreased on adding the Hg2+ and Ag+ ion respectively We propose that the addition of the metal ion could be a convenient and accurate strategy to detect the mismatched base pair in the heteroduplex This novel strategy might make the heteroduplex analysis easy and eventually lead to better SNP detectionThis research was partly supported by the Casio Science Promotion Foundation Iketani Science and Technology Foundation Nakatani Foundation of Electronic Measuring Technology Advancement and Tateishi Science and Technology Foundation This work was also supported in part by GrantinAid for JSPS Fellows 08J07706 to TK from the Ministry of Education Science Sports and Culture of Japan
Keywords:
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Other Papers In This Journal:
- Synthesis, structures and magnetic properties of three metal-organic frameworks containing manganese(II)
- Synthesis and X-ray crystal structures of a series of 3d–4f lanthanide complexes: lanthanide(III)–copper(II) coordination polymers with 2,5-pyridinedicarboxylic acid ligand
- Intermediates in reactions of the diimine complex trisferrozineiron(II) with hydroxide or cyanide in binary aqueous solvent mixtures and in micelles
- A novel series of nickel(II)-dppe-arylazoimidazole complexes. Synthesis and spectroscopic characterization
- Gold(I)-Gold(III)-4,4′-bpy-phosphine complexes: Synthesis and multinuclear NMR study
- A copper(II) complex of the Schiff base from l -valine and 2-hydroxy-1-naphthalidene plus 1,10-phenanthroline: synthesis, crystal structure, and DNA interaction
- Biophysical, spectroscopic and biochemical investigation of DNA–Cu(II)-GSH interactions
- Kinetics and mechanism of substitution of iron(II)–triazine complexes by 1,10-phenanthroline
- Three coordination polymers constructed from bis(imidazol-l-yl-methyl)benzene: syntheses, structures, and third-order nonlinear optical properties
- Synthesis and photophysical characterization of ruthenium(II) and platinum(II) complexes with bis-pyridylethynyl-phenanthroline ligands as a metalloligand
- DNA binding and photocleavage properties of two mixed-ligand oxovanadium complexes
- Infrared and Raman spectroscopic characterization of the phosphate mineral kosnarite KZr 2 (PO 4 ) 3 in comparison with other pegmatitic phosphates
- Outer-Sphere electron-transfer and the effect of linkage isomerism in the titanium(III) reduction of nitro-pentacyanocobaltate(III) and nitro-, and nitrito-pentaamminecobalt(III) complexes in aqueous acidic media
- A series of trinuclear sandwich-like cyanide-bridged iron(III)-manganese(II) complexes: synthesis, crystal structures, and magnetic properties
- Synthesis and physico-chemical studies on transition metal complexes of symmetric bis -Schiff base ligands
- Exchange coupling in di-μ-hydroxo-bridged chromium(III) dimers
- Inner sphere oxidation of N , N ′-ethylenebis(isonitrosoacetyleacetoneimine)copper(II) by N -bromosuccinimide in aqueous weakly acidic solutions
- Initial state and transition state solvation in substitution at the square-planar complexes, [PtCl 4 ] 2− and [Pd(Et 4 dien)Cl] +
- Catalysis of the coupling reaction of aryl chlorides with bis(pinacolato)diboron by tricyclohexylphosphine-cyclopalladated ferrocenylimine complexes
- Ruthenium(III) – catalyzed oxidative cleavage of p -aminoazobenzene by chloramine-B in alkaline medium and uncatalyzed reaction in acid medium: spectrophotometric kinetic and mechanistic study
- An oxovanadium(IV) complex of an l -serine Schiff base and 1,10-phenanthroline: synthesis, crystal structure, and DNA and albumin-binding properties
- Mössbauer study of some iron(III) complexes with urea type ligands and the crystal structure of hexakisdimethylureairon(III) perchlorate
- Preparation and characterization of Co(II), Ni(II) and Cu(II) complexes of a ligand containing 1,3,4-thiadiazole groups
- Syntheses, crystal structures, and magnetic and fluorescence properties of three complexes of mixed flexible N,N′-bis(3-pyridylmethyl)amine and rigid pyromellitate ligands
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