Hexaploid H1 ES cells established from octaploid

Authors: Kohzaburo FujikawaYamamoto Minoru Miyagoshi Xianwen Luo Hiroko Yamagishi

Publish Date: 2010/12/29

Volume: 24, Issue: 1, Pages: 13-20

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Abstract

Hexaploid H1 ES cells 6H1 cells were established from octaploid H1 cells 8H1 cells as were pentaploid H1 cells 5H1 cells 6H1 cells were compared with 5H1 cells The number of chromosomes of 6H1 cells was 115 20 more than the 95 of 5H1 cells The durations of G1 S and G2/M phases of 6H1 cells were 3 7 and 6 h respectively almost the same as those of 5H1 cells The cell volume of 6H1 cells was equivalent that of 5H1 cells The morphology of 6H1 cells was flattened circular cluster different from the spherical cluster of 5H1 cells 6H1 cells exhibited alkaline phosphatase activity as well as 5H1 cells The DNA content of 6H1 cells was stable and maintained for 300 days of culturing the same as that of 5H1 cells The DNA stability of 6H1 cells was explained using a hypothesis concerning the DNA structure of polyploid cells because the asymmetric configuration of homologous chromosomes in 6H1 cells inhibited chromosome loss

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