The study of oleanolic acid on the estrodiol produ

Authors: Qian Wan Hua Lu Xia Liu Shangmian Yie Junbei Xiang Zouying Yao

Publish Date: 2014/07/16

Volume: 28, Issue: 1, Pages: 5-13

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Abstract

The women during the menopause period have an increased tendency for the obesity which represents the more fat production than during the premenopausal period Although this is not beneficial overall it could provide a compensatory source for the estrogen production for the menopausal women So it would be meaningful to find an agent that could inhibit the fat production while does not disturb the total estrogen production by fat tissues In the present study the effect of oleanolic acid OA on the fat production and the total estrogen production of the differentiating mouse preadipocyte 3T3L1 as well as the mechanisms behind those effects were preliminarily investigated The cell line 3T3L1 was chosen as the model cell because it is usually used for the research about the obesity During the induced differentiation of 3T3L1 cells cells were intervened continuously with OA The fat production was determined with the oil red staining assay and the total estrogen production was measured with the ELISA assay Finally the expression patterns for important genes of the fat production and the estrogen production were studied respectively with the realtime fluorescence quantitative PCR qPCR The results showed that for the differentiating 3T3L1 cells OA could significantly inhibit the fat production and did not disturb the total estrogen production significantly In the mechanism studies OA was found to significantly downregulate ACC the key gene for fat synthesis which could explain the inhibitory effect of OA on the fat production OA was also found to significantly upregulate CYP11A1 CYP17 CYP19 the key genes for the estrogen synthesis and significantly downregulate CYP1A1 the key gene for the estrogen decomposition which preliminarily explained the lack of the effect of OA on the total estrogen production In conclusion OA was found able to inhibit the fat production while maintaining the total estrogen level and the mechanisms for the above findings were preliminarily clarified which suggests that OA may be useful to treat the menopausal obesityWe sincerely thank Madam Peng Wenzhen of Sichuan University for the cultured cells and the cell culturing The National Program on Key Basic Research Project of China 973 Program No 2010Cb530403 Experimental Technology Program of Chengdu University of Traditional Chinese Medicine No 063003 and the University Foundation of Chengdu University of Traditional Chinese Medicine No ZRMS201248 supported this workWe declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work There is no professional or other personal interest of any nature or kind in any product service and/or company that could be construed as influencing the position presented in or the review of the manuscript entitled ‘‘the study of oleanolic acid on the estradiol production and the fat production of mouse preadipocyte 3T3L1 in vitro’’

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