Journal Title
Title of Journal: Human Cell
|
|
|
|
|
|
|
|
|
|
Authors: Zahra Vojdani Sima Tavakolinejad Tahereh TalaeiKhozani Tahereh Esmaeilpour Manuchehr Rasooli
Publish Date: 2011/02/18
Volume: 24, Issue: 1, Pages: 35-42
Abstract
Cell transplantation shows potential for the treatment of cardiac diseases Embryonic stem cells cord blood and mesenchymal stem cells have been suggested as sources for transplantation therapy Because of some technical limitations with the use of stem cells transdifferentiation of fully differentiated cells is a potentially useful alternative We investigated whether human peripheral blood cells could transdifferentiate into cardiomyocyte Transdifferentiation was induced in a human B lymphocyte cell line Raji Cardiomyocyte extract was prepared from adult mouse cardiomyocytes The cells were treated with 5aza2deoxycytidine and trichostatin A permeabilized with streptolysin O and exposed to the mouse cardiomyocyte extract They were cultured for 10 days 3 weeks and 4 weeks Cardiomyocyte markers were detected with immunohistochemistry and flow cytometry Immunocytochemistry revealed that some cells expressed myosin heavy chain αactinin and cardiac troponin T after 3 and 4 weeks Flow cytometry confirmed these data In cells exposed to trichostatin A and 5aza2deoxycytidine and permeabilized in the presence of the cardiomyocyte extract troponin T expression was seen in 353 of the cells and 311 of them expressed αactinin After exposure to the cardiomyocyte extract some permeabilized cells adhered to the plate loosely however the morphology did not change significantly and they continued to show a rounded shape after 4 weeks Our treated lymphocytes expressed cardiomyocyte markers Our results suggest that lymphocytes may be useful in future research as a source of cells for reprogramming proceduresThe authors thank the ViceChancellor for research of the Shiraz University of Medical Sciences for support through grant no 884578 and Ms L Rohani for excellent technical support We also thank K Shashok Author AID in the Eastern Mediterranean for improving the use of English in the manuscript and M Gholami at the Center for Development of Clinical Research of Nemazee Hospital for research assistance This study formed part of the work done by S Tavakolinejad for a thesis
Keywords:
.
|
Other Papers In This Journal:
|