Authors: Ali Abdel Aziz ElMashad Heba Ibrahim Mohamed
Publish Date: 2011/07/06
Volume: 249, Issue: 3, Pages: 625-635
Abstract
Soil salinity is one of the most severe factors limiting growth and physiological response in Vigna sinensis plants Plant salt stress tolerance requires the activation of complex metabolic activities including antioxidative pathways especially reactive oxygen species and scavenging systems within the cells which can contribute to continued growth under water stress The present investigation was carried out to study the role of brassinolide in enhancing tolerance of cowpea plants to salt stress NaCl Treatment with 005 ppm brassinolide as foliar spray mitigated salt stress by inducing enzyme activities responsible for antioxidation eg superoxide dismutase peroxidase polyphenol oxidase and detoxification as well as by elevating contents of ascorbic acid tocopherol and glutathione On the other hand total soluble proteins decreased with increasing NaCl concentrations in comparison with control plants However lipid peroxidation increased with increasing concentrations of NaCl In addition to the high concentrations of NaCl 100 and 150 mM decreased total phenol of cowpea plants as being compared with control plants SDSPAGE of protein revealed that NaCl treatments alone or in combination with 005 ppm brassinolide were associated with the disappearance of some bands or appearance of unique ones in cowpea plants Electrophoretic studies of αesterase βesterase polyphenol oxidase peroxidase acid phosphatase and superoxide dismutase isoenzymes showed wide variations in their intensities and densities among all treatments
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