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Title of Journal: Protoplasma

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Abbravation: Protoplasma

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Springer Vienna

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DOI

10.1016/0306-3747(92)90143-N

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1615-6102

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Preapoptotic activity of aqueous extracts of Emp

Authors: Neethu Kannan Bhagyanathan John Ernest Thoppil
Publish Date: 2015/10/22
Volume: 253, Issue: 6, Pages: 1433-1438
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Abstract

Cynanchum sarcomedium Meve Liede is a member of Apocynaceae seen in dry and rocky areas The present study highlights the cytotoxic potential of C sarcomedium mediated by apoptosis on cells of Allium cepa and human red blood cells RBCs Cytogenetic changes in A cepa and in situ visualization of cell death were revealed through acetocarmine and Evans blue staining techniques Quantitative estimation of cell death was carried out at 600 nm in a spectrophotometer Membrane characteristics of RBC in response to the treatment were evaluated by MayGrünwaldGiemsa staining and scanning electron microscopy SEM Cell membrane damage is a major factor for assessing apoptosis which is observed in the present study 9091  Cell shrinkage cytoplasmic fragmentation condensed chromatin and presence of apoptotic bodies were the common cytological changes in A cepa associated with apoptosis Blebs in RBC evidenced by SEM revealed the membrane damage potential of the plant Results obtained hereby suggest that the plant is an effective source to be used in toxicological studies and anticancer therapy

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