Authors: Veronika Zelinová Igor Mistrík Ján Pavlovkin Ladislav Tamás
Publish Date: 2013/01/17
Volume: 250, Issue: 5, Pages: 1057-1065
Abstract
The purpose of this study was to analyse the alterations of glutathione peroxidase GPX expression and activity during the recovery period after a shortterm treatment of barley root tip with cadmium Cd and hydrogen peroxide H2O2 The transcript level of GPX increased as early as 1 h and GPX activity 3 h after shortterm treatment independently of Cd concentration In 15 μM Cdtreated roots its expression reached a peak within 2 h and sustained until 3 h after which it gradually declined After 6 h of shortterm Cd treatment the activity of GPX was the highest in the 15μM Cdtreated roots At higher Cd concentrations the activity of GPX was lower than in 15 μM Cdtreated roots but still higher than in control roots A considerable increase in H2O2 production was observed even after only 1 h of shortterm exposure of roots to 30 and 60 μM Cd while after 15 μM Cd exposure its production increased 3 h after the treatment Lipid peroxidation increased even 1 h after shortterm treatment in a Cd concentrationdependent manner A considerable decrease of GPX activity was observed after the exposure of roots to H2O2 or tbutyl hydroperoxide in a concentrationdependent manner despite that its expression increased even 1 h after shortterm treatment Presumable under high acute Cd stress rapid accumulation of H2O2 leads to the disturbance of basal metabolic processes affecting also GPX activity In contrast high GPX activity under moderate Cd stress maintains cell function despite the high rate of H2O2 metabolism in root tip
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