Visualization of the Superparamagnetic Iron Oxide

Authors: Hideo Shichinohe Satoshi Kuroda Kohsuke Kudo Masaki Ito Masahito Kawabori Michiyuki Miyamoto Mitsuhiro Nakanishi Satoshi Terae Kiyohiro Houkin

Publish Date: 2011/12/13

Volume: 3, Issue: 1, Pages: 99-106

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Abstract

Recent studies have elucidated that transplantation of the bone marrow stromal cells BMSC has therapeutic potential for the central nervous system CNS disorders However no imaging modalities have been established to track the engrafted cells in the CNS in clinical situation This study aimed to investigate the ability of magnetic resonance imaging MRI to visualize the BMSC labeled with superparamagnetic iron oxide SPIO The BMSC of mice were labeled with SPIO Various numbers of the cells were injected into the agar phantom and were visualized using a 30T MR apparatus The SPIOlabeled cells were injected into the temperaturesensitive gelation polymer TGP hydrogel and were cultured for 7 days They were also visualized just after the injection and at 7 days postinjection After a 7day culture they were stained with Turnbull blue technique T2 T2 and susceptibilityweighted imaging could identify minimally 1000 cells in the agar or TGP hydrogel although it was difficult to quantify their number on MRI All of these sequences could track the SPIOlabeled BMSC for at least 7 days when injected into the TGP Turnbull blue staining revealed the survival and proliferation of the SPIOlabeled BMSC in the TGP for 7 days The findings strongly suggest that the SPIO labeling may enable to track minimally 1000 cells engrafted in the CNS on clinical MR apparatus These data would be valuable to consider the application of imaging technique into cell transplantation therapy for CNS disorders

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