Bipyridine an Iron Chelator Does Not Lessen Intr

Authors: Jayalakshmi Caliaperumal Shannon Wowk Sarah Jones Yonglie Ma Frederick Colbourne

Publish Date: 2013/08/06

Volume: 4, Issue: 6, Pages: 719-728

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Abstract

Iron chelators such as the intracellular ferrous chelator 22′bipyridine are a potential means of ameliorating ironinduced injury after intracerebral hemorrhage ICH We evaluated bipyridine against the collagenase and wholeblood ICH models and a simplified model of ironinduced damage involving a striatal injection of FeCl2 in adult rats First we assessed whether bipyridine 25 mg/kg beginning 12 h postICH and every 12 h for 3 days would attenuate nonheme iron levels in the brain and lessen behavioral impairments neurological deficit scale corner turn test and horizontal ladder 7 days after collagenaseinduced ICH Second we evaluated bipyridine 20 mg/kg beginning 6 h postICH and then every 24 h on edema 3 days after collagenase infusion Body temperature was continually recorded in a subset of these rats beginning 24 h prior to ICH until euthanasia Third bipyridine was administered as per experiment 2 after wholeblood infusion to examine tissue loss neuronal degeneration and behavioral impairments at 7 days poststroke as well as body temperature for 3 days poststroke Finally we evaluated whether bipyridine 25 mg/kg given 2 h prior to surgery and then every 12 h for 3 days lessens tissue loss neuronal death and behavioral deficits after striatal FeCl2 injection Bipyridine caused a significant hypothermic effect maximum drop to 346 °C for 2–5 h after each injection in both ICH models however in all experiments bipyridinetreated rats were indistinguishable from vehicle controls on all other measures eg tissue loss behavioral impairments etc These results do not support the use of bipyridine against ICH

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