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Title of Journal: J Am Soc Mass Spectrom

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Abbravation: Journal of The American Society for Mass Spectrometry

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Springer-Verlag

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DOI

10.1007/s10243-006-0097-9

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1879-1123

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Application of Probe Electrospray Ionization Mass

Authors: Mridul Kanti Mandal Kentaro Yoshimura Lee Chuin Chen Zhan Yu Tadao Nakazawa Ryohei Katoh Hideki Fujii Sen Takeda Hiroshi Nonami Kenzo Hiraoka
Publish Date: 2012/08/25
Volume: 23, Issue: 11, Pages: 2043-2047
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Abstract

We have examined several combinations of solvents with the aim of optimizing the ionization conditions for molecular diagnosis of malignant tumours by PESIMS Although the best conditions may depend on the actual species in the sample the optimal conditions for renal cell carcinoma RCC were achieved by using alcohols PESIMS successfully delineated the differential expression of phospholipids PCs and triacylglycerols TAGs in noncancerous and RCC tissues by using these solvent systems This study paves the way for the application of PESIMS in medical samplesThe application of mass spectrometry MS to medicine has a relatively long history dating back to 1980s when gas chromatography mass spectrometry GCMS had been applied in the kinetic analysis of bile acid in patients with Crohn’s disease 1 Subsequently the development of new ionization techniques such as electrospray ionization ESI 2 and matrixassisted laser desorption ionization MALDI 3 have accelerated the application of MS in medicine 4Recently desorption electrospray ionization mass spectrometry DESIMS has been applied successfully to lipid characterization 5 and biological tissue imaging 6 7 Takáts et al reported a promising technique named “rapid evaporative ionization mass spectrometry REIMS” which allows realtime identification of tissue properties during surgery 8To address clinical requirements we have developed probe electrospray ionization PESI 9 10 which uses a solid needle as a sampling probe and an ESI emitter PESIMS has been applied to the direct analysis of biological samples and was validated as a useful method for measuring mass spectra without any sample pretreatment 11 12 Recently we have also applied PESIMS to realtime analysis of organs in living mice and showed the usefulness in distinguishing differences in lipid composition of hepatocytes between normal and steatotic mice All mice used in this study tolerated the experimental procedure of PESIMS because of its low invasiveness and survived for months until being sacrificed for further analyses 13In our previous work 10 it was found that a singleshot PESIMS using a 05 mmdiameter titanium Ti wire could virtually ionize all the analytes contained in a droplet at the needle tip The analytes undergo sequential and exhaustive ionization according to their surface activities and these phenomena help alleviate the ionsuppression effect that is inherent to capillarybased ESI in which sample solution is continuously supplied to the capillaryTo examine the solubility of lipids we have used water and several organic solvents for lipids extraction in human cancer tissues Under these conditions renal cell carcinoma RCC is characterized by accumulation of triacylglycerol TAG 14 15 which is a good model for proving the usefulness of PESIMS

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  2. On the Efficiency of NHS Ester Cross-Linkers for Stabilizing Integral Membrane Protein Complexes
  3. Dynamic Interchanging Native States of Lymphotactin Examined by SNAPP-MS
  4. Quantitative Assessment of Protein Structural Models by Comparison of H/D Exchange MS Data with Exchange Behavior Accurately Predicted by DXCOREX
  5. Reflections on Charge State Distributions, Protein Structure, and the Mystical Mechanism of Electrospray Ionization
  6. CYCLONE—A Utility for De Novo Sequencing of Microbial Cyclic Peptides
  7. Mass Spectrometry-Based Quantification of Pseudouridine in RNA
  8. Statistical Examination of the a and a + 1 Fragment Ions from 193 nm Ultraviolet Photodissociation Reveals Local Hydrogen Bonding Interactions
  9. Perspective on Electrospray Ionization and Its Relation to Electrochemistry
  10. Untargeted Metabolomics Strategies—Challenges and Emerging Directions
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  14. Charge Detection Mass Spectrometry for Single Ions with an Uncertainty in the Charge Measurement of 0.65 e
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  16. Probing the Electron Capture Dissociation Mass Spectrometry of Phosphopeptides with Traveling Wave Ion Mobility Spectrometry and Molecular Dynamics Simulations
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