Authors: Tapas Mitra G Sailakshmi A Gnanamani A B Mandal
Publish Date: 2012/10/11
Volume: 20, Issue: 4, Pages: 647-661
Abstract
Dicarboxylic acids DCAs a precursor for many polyamides and polyester preparations may also find applications in the preparation of highperformance biopolymer material for tissue engineering research In the present study collagen a natural biopolymer when mixed with the chosen DCAs through ionic interaction between R–COO− and –NH3 + dissolution as well as stabilization of collagen was achieved which obviates the use of carbodiimide activation of –COOH groups of DCA The DCA engineered collagen biopolymer material obtained in the form of 3D scaffolds was subjected to evaluate its high performance through in vitro and in vivo studies Results on FTIR CD TGA DSC SEM mechanical properties docking simulation studies and binding energy calculations explored the interactions and the in vitro cytotoxicity assays and in vivo wound healing studies explored and authenticated the performance of the DCA engineered biopolymer material and suggested the option for tissue engineering researchOne of the authors Mr Tapas Mitra acknowledges CSIR New Delhi for the financial assistance provided in the form of CSIRSRF The authors wish to thank Dr Swaraj Sinha and Dr Suvro Chatterjee AuKBC research centre MIT Campus Anna University Chennai for their help and extending the lab facility to perform cell lines studies
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